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Objective:To analyze the characteristics of thyroid diameter and volume of children aged 8 - 10 years in Gansu Province, and to provide a basis for standardizing B-ultrasound examination of children's thyroid volume and establishing a normal reference value of children's thyroid volume.Methods:The data of thyroid volume by B-ultrasound and urinary iodine determination of children aged 8 - 10 years in Gansu Province were from Institute of Endemic Disease, Gansu Center for Disease Control and Prevention. Based on the measured data of thyroid B-ultrasound of 8 - 10 years children in Gansu Province, the characteristics and relationship between thyroid diameter and volume were analyzed by nonparametric test.Results:The medians of urinary iodine of 8, 9, 10 years children ( n = 500, 503, 496) were 166.62, 167.16 and 178.78 μg/L, respectively. The length, width, thickness and volume of the left and right lobes of the thyroid in children were skewed distribution. There was a positive correlation between the left and right diameters (length, width and thickness) and between left and right thyroid volumes ( r = 0.76, 0.85, 0.72, 0.88, P < 0.001). There was no significant difference in the six diameter lines and volume values of thyroid between genders ( P > 0.05), but there was significant difference between ages ( P < 0.001). The means thyroid volume of 8, 9 and 10 years children were 2.6, 2.9 and 3.2 ml, respectively, the medians were 2.5, 2.7 and 3.0 ml, respectively, and the 97th percentile ( P97) were 4.4, 4.9 and 6.5 ml, respectively. The thyroid volume of 10 years group was higher than the current national standard (6.0 ml). The median ranges of thyroid volume of children aged 8, 9 and 10 years at the county level were from 1.8 ml to 4.1 ml, from 1.8 ml to 4.3 ml and from 1.9 ml to 4.4 ml, respectively. There was significant difference in thyroid volume of children at the county level in all age groups ( P < 0.05). Conclusions:Compared with the national standard, the P97 value of thyroid volume of children aged 8 - 10 years in Gansu Province is slightly different. The reference value of thyroid volume should be formulated according to age, and a unified reference value can be used for different genders at the same age.
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Objective:To investigate the iodine nutritional status of population in Gansu Province, and to provide a scientific basis for policy adjustment of relevant departments.Methods:In 2019, a cross-sectional survey was conducted in 87 monitoring units in 86 counties (districts and cities) of 14 cities (prefectures) in Gansu Province for iodine deficiency disorders (IDD). Instant urine samples and salt samples of children aged 8 to 10 years and pregnant women were collected for detection of urinary iodine and salt iodine. B-ultrasound was used to check the thyroid volume of children in 40 monitoring units.Results:A total of 17 772 children aged 8 to 10 years were investigated. The median urinary iodine (MUI) of children was 197.4 μg/L, the MUI of 14 cities (prefectures) ranged from 160.6 to 233.4 μg/L. There were statistically significant differences in MUI among children of different genders and ages ( P < 0.001). Totally 8 269 children were examined by B-ultrasound, the children's goiter rate was 1.4% (118/8 269). There were statistically significant differences in the thyroid volume among children of different genders and ages ( P < 0.05). A total of 8 605 pregnant women were investigated. The MUI of pregnant women was 178.6 μg/L, and the MUI of 14 cities (prefectures) ranged from 156.5 to 275.0 μg/L. There was no statistical significant difference in MUI among pregnant women in different pregnancies ( P > 0.05). A total of 26 377 samples of salt consumed by children and pregnant women were detected. The median salt iodine was 24.71 mg/kg. The coverage rate of iodized salt and the consumption rate of qualified iodized salt were 99.44% and 93.59%, respectively. Twenty-three counties (districts and cities) failed to meet the elimination standard of IDD in the single indicator. Conclusions:IDD is in a state of continuous elimination in Gansu Province; iodized salt supervision needs to be further strengthened; there is an imbalance of control measures between regions.
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Objective:To evaluate the iodine level of drinking water in different ecological regions in Gansu Province, and to provide a basis for preventing iodine deficiency disorders in accordance with local conditions.Methods:In May to July 2017, according to different topography, landforms and climatic conditions, Gansu Province was divided into six ecological regions: desert area, Hexi Corridor gobi area, Loess Plateau hilly area, Qinling Zhongshan gorge area, Gannan Plateau grassland and meadow area, and Loess Plateau gully area. One county was selected from each of the five districts in the east, west, south, north, and central areas in each ecological regions. Drinking water samples were collected in each county based on the township, and the water iodine content was determined by arsenic-cerium catalytic spectrophotometry.Results:A total of 4 037 water samples were collected in 527 townships of six ecological regions, the water iodine range was 0.10 - 97.60 μg/L, with a median of 2.10 μg/L. Among them, the medians water iodine of the six ecological regions were less than 40 μg/L, the difference between different ecological regions was statistically significant ( H = 1 007.440, P < 0.01); the unified centralized, partially centralized and dispersed water supply water samples were 157, 2 263 and 1 617, and the medians water iodine were 1.61, 2.42, and 1.83 μg/L, respectively, and there was statistically significant difference among different water supply methods ( H = 37.164, P < 0.01). Stratified by water supply method, the differences in water iodine between different ecological regions of the same water supply method were statistically significant ( H = 32.628, 580.630, 391.070, P < 0.01); stratified by ecological region type, the differences of different water supply methods in desert area, Hexi Corridor gobi area, Qinling Zhongshan gorge area, Gannan Plateau grassland and meadow area were statistically significant ( Z = - 2.770, H = 6.369, 9.936, 12.666, P < 0.05). Conclusions:The six ecological regions in Gansu Province are all iodine-deficient areas. The water iodine content varies among different ecological region types and different water supply methods. Surveys on water iodine content and the operating status of water supply facilities should be carried out regularly, and measures should be adjusted in time to prevent and control iodine deficiency disorders in accordance with local conditions.
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Objective To explore the iodine nutritional status of all people after the practice of new standard of edible iodized salt, to assess whether the new standard of edible iodized salt can satisfy the iodine nutrition need in different populations, and to provide a scientific basis for supplementation of iodine. Methods Before and after the implementation of new salt iodine concentration, in 2009 and 2015, a contrastive study was performed in 1 243 people(including 402 children aged 8 to 10 years,408 adults aged 18 to 45 years,211 pregnant women and 222 lactating women) who lived in urban and rural areas of Wuwei City. Random urine samples were collected to measure the urinary iodine in all populations and household salt samples were collected. Urinary iodine was detected by arsenic cerium catalytic spectrophotometry(WS/T 107-2006).The iodine level in salt was determined by direct titration(GB/T 13025.7-2012).Results Before and after the implementation of new iodine salt standard, the coverage of adequately iodized salt was consistently higher than 94%, the qualified rate of iodized salt was consistently higher than 96%,the median of iodized salt was reduced from 31.0 mg/kg to 26.9 mg/kg;the medians of urinary iodine of children aged 8 to 10 years, adults, pregnant and breastfeeding women were reduced from 290.84, 225.52,200.42 and 180.65 μg/L to 187.37,174.96,159.99 and 152.88 μg/L;the ratios of urinary iodine which was less than 100 μg/L in children aged 8 to 10 years, adults and breastfeeding women were risen from 5.15% (10/194), 12.57% (24/191) and 16.19% (17/105) to 10.58% (22/208), 15.81% (34/215) and 33.33% (39/117); the ratio of urinary iodine which was less than 150 μg/L in pregnant women was risen from 39.78%(37/93)to 43.22%(51/118);the ratios of urinary iodine between 100 and 200 μg/L in children aged 8 to 10 years and adults were risen from 15.46% (30/194) and 30.37% (58/191) to 44.23% (92/208) and 43.26% (93/215); the ratios of urinary iodine greater than 300 μg/L in children aged 8 - 10, adults, pregnant and breastfeeding women were reduced from 47.94%(93/194),34.55%(66/191),29.76%(26/93)and 28.57% (30/105) to 17.31% (36/208), 19.07% (41/215), 16.95% (20/118) and 11.97% (14/117). Conclusion After the implementation of new salt iodine concentration standard,the iodine nutrition level has decreased in all groups and better suited for children aged 8 to 10 years and adults,but the risk of iodine deficiency is increased in pregnant and breastfeeding women.
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Objective To investigate the scope and distribution of water iodine in centralized water supply projects,and provide a basis for taking appropriate control measures in Gansu Province.Methods Collected 2 copies of water samples of all the different water supply of centralized water supply project water in the province's 87 counties (cities,districts),the source water iodine of all centralized water supply projects was tested by the method of cerium sulfate catalytic spectrophotometry.The relationship between different water types and different iodine levels in deep well water were analyzed.Results Of the total 1 563 centralized water supply projects,the median of water iodine was 6.38 μg/L,the range was 0.00-182.10 μg/L,the rates were 62.0％ (969/1 563),30.1％ (471/1 563),6.9％ (108/1 563),0.5％ (7/1 563) and 0.5％ (8/1 563) in water iodine level of ＜ 10,10-＜ 50,50-＜ 100,100-＜ 150 and ≥ 150 μg/L.The surface water was 26.7％ (418/1 563),the median of water iodine was 4.42 μg/L,the groundwater was 73.3％(1 145/1 563),the median of water iodine was 8.10 μg/L,the iodine content of surface water was lower than that of the groundwater (Z =-10.089,P ＜ 0.01);in surface water,the rate of river,lake,reservoir and other water source was 55.7％ (233/418),7.7％ (32/418),21.5％ (90/418) and 15.1％ (63/418),and the median was 2.52,31.10,6.65 and 5.40 μg/L,the iodine content between surface water and water sources of different water sources was significant different (x2 =179.976,P ＜ 0.01);in the groundwater,the rate of deep well,shallow well and spring was 55.5％ (635/1 145),41.3％ (473/1 145) and 3.2％ (37/1 145),and the median was 17.90,3.66 and 4.18 μg/L,the iodine content in groundwater from different sources was significant different (x2 =357.346,P ＜ 0.01).Iodine content of different well depth was significantly different (x2 =288.959,P ＜ 0.01),there was a positive correlation between iodine content and well depth (r =0.364,P ＜ 0.01).Conclusions Iodine content of the surface water is generally lower in many water types in Gansu,iodine content is higher in deep well,and there is high iodine deep well water in local areas.It is recommended that water iodine be included in the population iodine nutrition assessment index.
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Objective To assess iodine nutritional status of lactating women and infants (0-2 years old) in six ecological regions of Gansu Province,and to provide a scientific basis for iodine supplementation of target people.Methods According to different topography and climate conditions,Gansu Province was divided into six ecological regions:the desert region,the Gobi region of Hexi Corridor,the hills and valleys region of Loess Plateau,the Zhongshan Valley of Qinling Mountains,the grassland meadow area of Gannan plateau and the Loess Plateaugully region.Totally 5 counties were selected from each ecological region,and each county was divided into five geographic locations (east,south,west,north and center).In each location,one township was identified and ten samples of lactating women and infants (0-2 years old) were selected randomly and 60 salt samples of residents were collected to determine iodine content.At the same time salt intake of 20 residents was surveyed in three townships as mentioned above.The water samples of all the centralized water supply projects were collected in each county,and one water sample of the decentralized water supply in ten townships with the largest population of each county from east,south,west,north and center locations was collected to determine iodine content.One soil sample was collected to determine iodine content in east,south,west,north and center townships of each county.The urinary iodine and water iodine levels were measured with the method for determination of iodine in urine by As3+-Ce4+ catalytic spectrophotometry (WS/T 107-2006).The direct titration method among thegeneric methods for iodide testing in salt production industry (GB/T 13025.7-2012) was used to determine the salt iodine level,and the arbitration method was adopted for quantitative determination in the case of Sichuan salt or other special salts and the salt intake was estimated based on three day weighed food record.The soil iodine was determined using the amplified colorimetric method.Results Totally 1 476 and 1 461 urine samples of lactating women and infants were collected,and the total urine iodine medians of lactating women and infants (0-2 years old) were 149.05 and 151.34 μg/L,respectively,the urine iodine median of lactating women and infants in each ecological region was at the 100-199 μg/L appropriate level.Not weaning infants urine iodine (155.32 μg/L) was higher than that of the weaning infants (146.30 μg/L),the difference was significant statistically (Z =-2.808,P ＜ 0.05).There was a correlation between urine iodine of not weaning infants aged 0-2 years old and breastfeeding women (r =0.133,P ＜ 0.01),and there was no correlation between weaning infants and breastfeeding women (r =0.045,P ＞ 0.05).About 9 008 salt samples were collected,and the rates of qualified iodized salt intake in the six ecological regions were all higher than 93％.The daily salt intake per person ranged from 4.5 g to 12.6 g,in which the Loess Plateaugully region was the highest with 12.6 g and the grassland meadow area of Gannan plateau was the lowest with 4.5 g.The water iodine median of 1 513 water samples was 3.3 μg/L.The water iodine medians of the rest 4 ecological regions were all less than 5 μg/L except for the desert region (42.4 μg/L) and the Loess Plateau-gully region (30.0 μg/L) of which the median of water iodine was higher than 20 μg/L.The soil iodine median of 155 soil samples was 127 μg/kg,in which the desert region was the lowest with 78 μg/kg and the grassland meadow area of Gannan plateau was the highest with 218 μg/kg.Conclusion Lactating women and infants are in good iodine nutritional status and the present iodized salt concentration can meet the demand of iodine nutrition of lactating women and infants well.
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Objective To study the iodine nutritional status and thyroid function of adults in urban and rural areas of Wuwei City Gansu Province.Methods A cross-sectional survey was performed in 200 adults aged 18-45 who had lived more than six months in Wuwei City from April 2009 to January 2010.A random urine and fasting blood samples were collected.Urinary iodine content was measured with arsenic cerium catalytic spectrophotometry.The thyroid-stimulating hormone (TSH),free thyroid hormone (FT4) and three free triiodothyronine (FT3) were quantified by direct chemiluminescence immunoassay.Thyroglobulin antibodies (TGAb) and thyroid microsomal antibodies (TMAb) were detected by radioimmunoassay.Results A total of 99 and 98 copies of urine samples of urban and rural groups were tested,respectively.The median of urinary iodine of urban and rural groups was 189.0 and 258.2 μg/L,respectively,and rural group was higher than urban group (Z =-4.020,P ＜ 0.01).A total of 104 and 95 copies of blood samples of urban and rural groups were detected; mean value of FT4 in urban group [(16.8 ± 3.0)pmol/L] was higher than that of rural group [(15.4 ± 2.4)pmol/L,t =3.539,P ＜ 0.01].The positive rates of TGAb of urban and rural groups were 11.5％ (12/104) and 15.8％ (15/95),respectively; the positive rates of TMAb of urban and rural groups were 13.5％ (14/104) and 14.7％ (14/95),respectively; the positive rates of TGAb of male and female were 5.5％ (6/109) and 23.3％ (21/90),respectively; the positive rates of TMAb of male and female were 6.4％ (7/109) and 23.3％ (21/90),respectively.The positive rates of TGAb and TMAb were lower in male than in female (x2 =13.362,11.661,all P ＜ 0.01).The rates of thyroid function disorders of urban and rural groups were 16.3％ (17/104) and 8.4％ (8/95),of male and female 11.9％ (13/109) and 13.3％ (12/90),respectively.Subclinical hypothyroidism in all thyroid function disorders was the most common,which was 14.4％ (15/104) and 7.4％ (7/95) in urban and rural groups,11.0％ (12/109) and 11.1％ (10/90) in male and female,respectively.Conclusions Iodine nutrition level is appropriate for adults in urban areas,but in rural areas iodine nutrition is high or excessive; subclinical hypothyroidism in all thyroid function disorders is the most common; we should be concerned about the risk of iodine overdose,especially the risk of illness in women.
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Objective To build a case database of endemic cretinism,investigate the distribution of existing cretinism,and provide suggestions for prevention of iodine deficiency disorders.Methods In 2011,an existing endemic cretinism case database was established in Gansu Province by verifying and registering the historical cases before 1985,and searching and diagnosing the cases between 1985-2011 in history cretinism areas and IDD high-risk areas according to endemic cretinism and endemic sub-clinical cretinism diagnosis (WS 104-104) and the IDD monitoring program of the central transfer fund at the same time,and analysis the distribution of existing cretinism.Results In 1985 and 2011,cretinism areas were 53 and 41 in Gansu Province,and the endemic cretinism cases werel9 696 and 4 715,respectively;18 cases of endemic cretinism were born between 1985 to1997,7 new cases were born after 1997;nerve cretinism,myxedematous and mixed types among existing cases were 58.8％ (2 766/4 708),9.8％ (461/4 708) and 31.4％ (1 481/4 708),males and females were 69.3％ (3 261/4 708)and 30.7％ (1 481/4 708),respectively.Cases older than 45 accounted for 73.2％ (3 444/4 708);the 7 new cases were all nerve cretinism and 5 cases distributed in ethnic minority areas.Conclusions There are potential dangers of endemic cretinism in the high-risk areas of iodine deficiency disorders due to multiple factors in Gansu Province;the nervous cretinism is the majority which is caused by insufficient iodine intake in early maternal pregnancy.To prevent new endemic cretinism,it is necessary to strengthen measures of emergency iodine supplementation for pregnant women and nursing mothers in the high-risk areas.
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Objective To understand the iodine nutrition and thyroid function of different population in urban and rural areas of Gansu province and provide evidence for iodine supplementation.Methods A cross-sectional survey was performed in urban and rural children,adults,pregnant women and breastfeeding women in Wuwei from April 2009 to January 2010.Urine and fasting blood samples were collected from the subjects.Urine iodine content was measured with arsenic cerium catalytic spectrophatometry.The thyroid-stimulating hormone (TSH),free thyroid hormone (FT4) and three free triiodothyronine (FT3) were detected by using direct chemiluminescence immunoassy.Non parametric test was used to compare the urinary iodine and TSH group.t test was used to compare FT4,FT3.x2 test was used to compare the rate.Results The medians of urinary iodine level (μg/L) were 358.6,189.0,255.4 and 239.5 in urban children,adults,pregnant women and breastfeeding women and 387.6,258.5,172.8 and 215.3 in rural children,adults,pregnant women and breastfeeding women respectively.The median of urinary iodine of urban adults was significantly lower than that in rural adults (Z=-4.020,P=0.000) and the medians of urinary iodine level of urban pregnant women was higher than that in rural pregnant women (Z=1.424,P=0.035).The mean valuc of FT3 in rural pregnant women and breastfeeding women were higher than that in urban groups (t=-3.933,P=0.000;t=-2.259,P=0.026).The mean value of FT4 in urban adults was higher than that in rural adults (t=3.539,P=0.001).The positive rate of TGAb and TMAb in rural pregnant women and breastfeeding women were 43.6％,56.4％ and 33.3％,35.6％,respectively,which were higher than those in urban groups.Subclinical hypothyroidism in all thyroid function disorders was common in all the groups.No statistical significant difference in all thyroid function disorders were found in different population.Conclusion The iodine nutrition were in good status in both urban population and rural population,the children' s iodine nutrition was surplus.Statistical differences existed in iodine nutrition status or thyroid hormone level between urban and rural adults,pregnant women and breastfeeding women.
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Objective To assess the iodine nutrition and thyroid function of pregnant women during different periods of pregnancy,to provide evidence for guiding iodine supplementation for them.Methods A cross-sectional survey was performed in 215 pregnant women in Yongjing couty from May to June 2013.Samples of blood and random urine were collected,and serum thyrotrophin (TSH),free triiodothyronine (FT3),free thyroxine (FT4),anti-thyroid peroxidase (anti-TPO),antithyroglobulin (anti-TG) and urinary iodine were measured.Results The medians of urinary iodine from the three groups of pregnant women (first,second and third trimester) were 189.8 μg/L,152.5 μg/L and 144.9 μg/L respectively.With the exception of pregnant women in the third trimester,the urinary iodine medians of pregnant women in the first and second trimesters were within the 150-249 μg/L range which was defined as optimal by WHO/UNICEF/ICCIDD.With the increase of gestational age,the level of FT3 decreased (P＜0.05),with the FT3 levels in the first trimester were higher than those in the second or third trimester (P＜0.05).The difference of TSH levels among the three groups of pregnant women was statistically significant (P＜0.01),with a U-shaped curve seen between the iodine TSH levels and the gestational age.The medians of anti-TG and anti-TPO appeared the lowest in the first trimester,and remained at a high level in women at second and third trimesters.Significant diffierence was seen in anti-TG,anti-TPO levels of the three groups of pregnant women (first,second and third trimester) (P＜0.01).The incidence of thyroidfunction disorder was 1.86％,including subclinical hypothyroidism accounted for 1.40％,and hypothyroidism accounted for 0.47％.The incidence of thyroidfunction disorder mainly appeared in the early pregnancy.Abnormal FT3,TSH,positive anti-TG and anti-TPO were mainly seen during early pregnancy.The changes of TSH,FT3,FT4,anti-TG and anti-TPO along with the changes of urine iodine levels were not obvious.Conclusion With the increase of gestational age,the incidence of iodine deficiency also increased among pregnant women.Abnormal thyroid hormones,TSH,positive anti-TG and anti-TPO were mainly existed in the early pregnancy.Programs as monitoring urinary iodine as well as thyroid function targeting all the pregnant women should be carried out.
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Objective To investigate the association of tumor necrosis factor-alpha (TNF-α) gene promoter region - 1031T/C and its combination with interleukin-6 (IL-6 ) gene promoter region -634C/G single nucleotide polymorphisms (SNP) with the genetic susceptibility to endometriosis.Methods Total of 432 endometriosis patients and 499 non-endometriosis women who had received an operation due to tubal ligation,tubal recanalization,laparoscopic hydrotubation,ovarian simple cyst and teratoma were collected and separated into endometriosis group and control group,that all cases were confirmed by operation and pathology.A case-control study was performed in endometriosis and control group to evaluate the association of these SNP with the susceptibility to endometriosis by using a fluorescent quantitative PCR-based high resolution melting ( HRM ) method.Results ( 1 ) TNF-α - 1031T/C genotype:the T and C of TNF-α - 1031T/C allele frequencies in the endometriosis group and control group were 79.2％ (684/864),20.8％ (180/864) and 81.8％ (816/998),18.2％ (182/998),respectively.The TT,TC and CC of TNF-α - 1031T/C genotype frequencies in the two groups were 63.7％ (275/432),31.0％ ( 134/432 ),5.3％ (23/432) and 66.5％ (332/499),30.5％ (152/499),3.0％ ( 15/499),respectively.There were no statistical significances in the TNF-α - 1031T/C alleles and genotypes distributions between the two groups ( P =0.158,P =0.186 ).( 2 ) TNF-α - 1031T/C and IL-6 - 634C/G conjoint genotypes:to research on the TNF-α - 1031T/C and IL-6 -634C/G genotypes for conjoint analysis,the TT + CC,TC + CC,CC +CC,TT + CG,TC + CG,CC + CG,TT + GG,TC + GG and CC + GG combination genotype frequencies in the two groups were 39.4％ ( 170/432 ),19.4％ ( 84/432 ),4.6％ ( 20/432 ),20.6％ ( 89/432 ),8.8％ (38/432),0.9％ (4/432),3.5％ (15/432),2.3％ (10/432),0.5％ (2/432) and 36.7％ ( 183/499),17.4％(87/499),1.4％ (7/499),26.1％ (130/499),10.4％ (52/499),1.2％ (6/499),3.8％ (19/499),2.6％ ( 13/499),0.4％ (2/499),respectively.There were no statistical significances in the combination genotypes distributions between the two groups ( P =0.107 ).As compared with carriers of TT + CC combination genotype,the endometriosis risk of carriers of CC + CC combination genotype enhanced 3.076 times ( 95％ CI:1.268 - 7.457,P =0.009 ),and the endometriosis risk of carriers of other combination genotypes were no statistical significances (all P ＞ 0.05 ).ConclusionsThe study demonstrates that there are no significant association between the SNP of TNF-α - 1031T/C and genetic susceptibility to endometriosis.However the results indicate that there are significant association betweengenetic susceptibility to endometriosis and the combination polymorphisms of TNF-α -1031T/C and IL-6- 634C/G.
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Aim To study the effect of chromomycin on MPP~+-induced dopaminergic neuronal apoptosis.Methods An apoptosis model induced by 1-methyl-4-phenylpridnium ion(MPP~+)was established in cultured fetal mesencephalic neurons in vitro.Dopaminergic neuronal apoptosis was detected by Hoechst 33258 staining and phospho-Tau levels were detected by immunofluorescence.Results 10 μmol·L~(-1) MPP~+ hyperphosphorylated Tau at Ser396 and induced dopaminergic neuronal apoptosis.Chromomycin increased the number of TH-positive cells via inhibiting Tau phosphorylation.Conclusion These results indicate thatchromomycin inhibits Tau phosphorylation at Ser396 and therefore protects dopaminergic mesencephalic neurons from apoptosis induced by MPP+,which suggests that Chromomycin may be used to cure neurodegenerative disorders such as Parkinson's disease in clinic.
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AIM: To explore the mechanism of nicotine against the apoptosis induced by colchicines in rat cortical neurons.METHODS: Cortical neurons were cultured from newborn Sprague-Dawley(SD) rats(less than 12 h).The rate of apoptosis was measured by Hoechst33258 fluorescence staining in the neurons,and the activity of Akt473 was analyzed by assay kit Akt473.RESULTS: The apoptosis of cortical neurons can be induced by 0.1 ?mol/L colchicine.The phosphorlation of Akt 473 decreased significantly(1/3 times of the control group,P